Lab Protocols
Molecular biology:
- Preparation of chemical compentent cells (E.coli)
- Plasmid isolation via P1, P2, P3
- Transformation of chemical competent cells
- Ni column chromatography for 6X his-tagged proteins
- Kenji Watanbe’s Never Fail Cloning Technology
- Gene expression in and protein extraction from E. coli
- F1 Medium for Cultivation of E. coli
- Electroporation of Myxococcus xanthus
- Thermal denaturation of protein by circular dichroism
Cell Culture:
Organic and Bioorganic:
Links
NEBcutter (restriction-digest
software)
Sequence Utilities
(get complement, reverse-direction, etc. for DNA sequence)
Encyclopedia of E. coli K12 Genes and Metabolism
Presentation/Posters
June 17, 2007 AGEP
Steve Houghton - Dihydropyran
formation versus oxetane formation in Laulimalide
May, 2007 Graduate Student's Symposium (GSS) Univ of Buffalo
Ata Pinto -
David Dixson -
Meng Wang - Understanding substrate
specificity of polyketide synthase thioesterase domain: Alanine scanning
of the substrate binding pocket
Burk Wilke - title
Steve Houghton - Dihydropyran
formation versus oxetane formation in Laulimalide
April, 2007 Mayfest at Syracuse
University
Mariya Ryabushkina - Comparison
of oxetane formation in macrolactone model systems
Steve Houghton - Dihydropyran
formation versus oxetane formation in Laulimalide
October, 2006 ACS NERM Binghamton, NY
Ata Pinto - SpiculoicAcid A: Understanding
its Biogenesis through its Biomimetic Total Synthesis
Ben Lundgren - Sialic Acid
Overproduction by Metabolic Engineering of Escherichia coli
Meng Wang - Site-Directed Mutagenesis
and Catalytic Activity Analysis of Thioesterase Domain of 6-deoxyerythronolide
B Synthase
Steve Houghton - Enhancing therapeutic protein production using a resin
bound c-glycoside sialidase inhibitor
Mariya Ryabushkina -
Laura Furst - title